RESEARCH AND PROFESSIONAL EXPERIENCE:
Concluding with present position, list, in chronological order, previous employment,
experience, and honors. Key personnel include the principal investigator and
any other individuals who participate in the scientific development or execution
of the project. Key personnel typically will include all individuals with
doctoral or other professional degrees, but in some projects will include
individuals at the masters or baccalaureate level provided they contribute
in a substantive way to the scientific development or execution of the project.
Include present membership on any Federal Government public advisory committee.
List, in chronological order, the titles, all authors, and complete references
to all publications during the past three years and the representative earlier
publications pertinent to this application. POSTDOCTORAL TRAINING:
1982-1984 Medical Resident, St. Michael’s Medical Center, Newark, NJ 1985
Chief Medical Resident, St. Michael’s Medical Center, Newark, NJ 1985-1988
Clinical Fellow, Allergy/Immunology, Mount Sinai Hospital, NY, NY 1988-1990
Postdoctoral Fellow, Immunobiology Institute, Mount Sinai School of Medicine,
NY, NY HONORS: 1983 Upjohn Achievement Award for Excellence in Medicine 1986
Fellows Award, American College of Allergists 1988-1990 Revson Fellow for
Biomedical Research, Mount Sinai Hospital, NY, NY 1993 Charles Newman Scholar,
Mount Sinai Hospital, NY, NY 1994-1999 Irma T. Hirschl Career Development
Award 1994-1999 First Award, National Institutes of Health
ACADEMIC APPOINTMENTS:
1988-1991 Instructor in Medicine, Mount Sinai School of Medicine 1991-1996
Assistant Professor of Medicine, Mount Sinai School of Medicine 1996-present
Associate Professor of Medicine and Immunology Research Projects Ongoing or
Completed During the Last Years “A Novel System to Study HIV-1- Monocyte Interactions”
Agency: National Cancer Institute Type: R-29 (CA66522, Years 1-5) Period (7/1/94-6/30/99)
This project studies monocyte-HIV-1 interactions utilizing humaqn macrophages
hybridomas. “Immunologic Mecxhanisms of Cow Milk Intolerance Program Project
Principle Investigator: Hugh Sampson MD, Project 2 Principle Investigator:
Kirk Sperber MD Agency: National Institute of Allergy and Infectious Disease
Type: PO-1 (AI 44236, Years 1-3) Period (9/1/99-8/31/03) This project investigates
kinetics of uptake and co-localization of cow milk peptides in intestinal
epithelial cells in cow milk allergic individuals. “Induction of Apoptosis
by HIV-1 Infected Monocytic Cells” Principle Investigator: Kirk Sperber MD
Agency: National Institute of Allergy and Infectious Disease Type R01 (1R01
AI 45343, Years 1-3) Period 12/1/99-11/30/02) This project seeks to identify
mechanisms by which monocytic cells induce apoptosis after HIV-1 infection.
PUBLICATIONS (selected from 55):
1. Sperber K, Bauer J, Pizzimenti A, Najfeld V, Mayer L. Identification of
subpopulations of human macrophages through the generation of human macrophage
hybridomas. Journal of Immunological Methods 129:31-40, 1990, 2. Alsteil L,
Sperber K, Mayer L. Cytokines in Alzheimer’s disease. Progress in Neuropsychopharmacology
and Biological Psychiatry 15: 481-495, 1991. 3. Shaked A, Sperber K, Mayer
L Stimulation of distinct T cell subsets in MLR using human macrophage hybridomas
differentially expressing class II antigens. Transplantation 52: 1068-1072,
1991. 4. Banerjee R, Bekesi G, Tarcsafalvi A, Sperber K, Deak G, Choi H-S,
Paronetto F, Holland JF, Acs G. Productive non-lytic HIV-1 replication in
a newly established biphenotypic cell line derived from a patient with myelodysplastic
syndrome. Proceedings of the National Academy of Science (USA) 89:996-1000,
1992. 5. Sperber K, Gollub E, Goswami SK, Kalb T, Mayer L, Marom Z. In vivo
detection of a novel macrophage-derived protein involved in the regulation
of mucus-like glycoconjugate secretion. American Review of Respiratory Diseases
146: (6) 1589-1597, 1992. 6. Stellrecht K, Sperber K, Pogo BGT. Activation
of the HIV-1 long terminal repeat by vaccina virus. Journal of Virology 66:2051-2056,
1992 7. Sperber K, Kalb TH, Stecher VJ, Banerjee R, Mayer L. Inhibition of
Human Immunodeficiency Virus Type 1 Replication by hydroxychloroquine in T
cells and monocytes. AIDS Research and Human Retroviruses. 9: (1):91-98, 1993.
8. Sperber K, Ogata S, Sylvester C, Aisenberg J, Chen A, Mayer L, Itzkowitz
S.A. Novel human macrophage-derived intestinal mucin secretagogue: implications
for the pathogenesis of inflammatory bowel disease. Gastroenterology 104:
1302-1309, 1993. 9. Sperber K, Quarishi H, Kalb T, Stecher V, Mayer L. Selective
regulation of cytokine secretion by hydroxycloroquine: inhibition of IL-1-alpha
and IL-6 in human monocytes and T cells. Journal of Rheumatology 20:803-808,
1993. 10. Sperber K, Hamrung G, Louie M, Kalb T, Banejee R, Mayer L. Progressive
impairment of monocytic function in HIV-1 infected human macrophage hybridomas.
AIDS Research and Human Retroviruses 9:657-668, 1993. 11. Sperber K, Sylvester
C, Kalb T, Goswami SK, Gollub SK, Gollub E, Druce H, Rutledge JL. Marom Z.
In vivo detection of a novel macrophage-derived protein involved in the regulation
of nasal mucus like glycoconjugate secretion. Journal of Allergy and Clinical
Immunology 92:581-588, 1993. 12. Zaghouani H, Anderson S, Sperber K, Mayer,
L, Kennedy R, Bona C. Induction of HIV-1 neutralizing antibodies in baboons
by immunization with recombinant V3 gp120. Proceedings National Academy of
Medicine 92:631-635, 1995. 13. Sperber K, Yoon C, Brusco C, Silverstein, Mayer
L. Patterns of cytokine secretion induced by superantigens in peripheral blood
lymphocytes, lamina propria lymphocytes, and intraepithelial lymphocytes.
Clinical and Diagnostic Laboratory Immunology 2:473-477, 1995. 14. Sperber
K, Chanez P, Bousquet J, Goswami S, Gollub E, Marom Z. Detection of a novel
macrophage-derived mucus secretagogue in asthmatic BALF. Journal of Allergy
and Clinical Immunology 95:868-876, 1995. 15. Yoo J, Chen H, Kraus T, Hirsch
D, Polyak S, Sperber K. Altered cytokine production and accessory cell function
in HIV-1 infected human macrophage hybridomas. Journal of Immunology 157:1313-11320,
1996. 16. Polyak S, Chen H, Hirsch D, George I, Hirschberg R, Sperber K. Impaired
class II expression and impaired antigen uptake in monocytic cells after HIV-1
infection. Journal of Immunology 159:2177, 1997. 17. Chen H, Yip YK, George
I, Sperber K. Chronically HIV-1 infected monocytic cells induce apoptosis
in co-cultured T cells Journal of Immunology 161:4257-4267, 1998. 18. Kishiyama
J, Valcer D, Cunningham-Rundles C, Sperber K et al. A multi-center, randomized
trial of high dose immune gammaglobulin for oral corticosteroid dependent
asthma. Clinical Immunology 91:126-133, 1999. 19. Eppinger T, Sperber K. Desensitization
to 5-fluorouracil. Allergy and Asthma Proc 20: 189-192, 1999. 20. Salik E,
Tyorkin M, Mohan S, Oei E, Kalb T, Sperber K. Patterns of accessory cell function
and antigen trafficking in respiratory epithelial cells. American J Respiratory
Cell and Molecular Biology 21:365-379,1999. 21. Boelaert J, Sperber K, Piette
J. Chloroquine exerts an additive in vitro anti-HIV type 1 effect when associated
with didansine and hydroxyurea. AIDS Research and Human Retroviruses 15: 1241-1247,
1999. 22. So AL, Pelton-Henrion, Small G, Becker K, Sperber K, Mayer L. Antigen
uptake and trafficking in human intestine epithelial cells. Digestive Diseases
Sciences 45:1130-1137, 2000. 23. So AL, Small G, Becker K, Sperber K, Mayer
L. Factors affecting antigen uptake by human intestinal epithelial cells.
Digestive Diseases Sciences 45:1451-1461, 2000. 24. Boelaert J, Sperber K,
Piette J. Chloroquine exerts an additive in vitro anti-HIV-1effect, when combined
to zidovudine and hydroxyurea. Biochemical Pharmacology 61:1531-1535, 2001
25. Rakoff-Nahoum S, Chen H, Kraus T, George I, Oei E, Sperber K. Regulation
of class II expression in monocytic cells after HIV-1 infection. Journal of
Immunology 167:2331-2342, 2001. 26. Savarino A, Gennero L, Chen H, Malavasi
F, Boelaert J, Sperber K. Anti-HIV-1 effects of chloroquine: mechanisms of
inhibition and spectrum of activity. AIDS 15:2221-2229, 2001.
Dr. Andrea Savarino was born in Turin on 24 November 1969.
Having graduated in Medicine and Surgery in Turin in October 1994, and specialized
in Infectious Diseases in Turin in October 1999, he has devoted most of his
professional activity to research work in the field of HIV infection.
His major areas of interest are exploring the mechanisms whereby the HIV virus
infects the target-cells and the experimental strategies aimed at inhibiting
viral replication.
He has carried out part of his research work in the United States, at the
University of Pittsburgh, PA, and at the Mount Sinai School of Medicine, New
York, NY. He is the author of numerous papers published on well-known international
journals.
Furthermore, he has taken part in a number of international conferences, both
as a speaker, in presenting his papers, and as the chairman, as well as acting
as referee for international scientific journals. The results of the researches
conducted by Dr. Andrea Savarino have also greatly attracted public opinion
and have been broadcasted through newspaper and TV interviews.
He currently carries out his research work at the Institute of Infectious
Disease Clinic of the “Università Cattolica del Sacro Cuore” in Rome and at
the Department of Medical Science of the “A. Avogadro” University in Eastern
Piedmont.
In addition, he continues to cooperate with the Department of Immunology of
the Mount Sinai School of Medicine in New York.